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. 2021 Aug 13;297(3):101079. doi: 10.1016/j.jbc.2021.101079

Figure 8.

Figure 8

NEPH1/NEPHRIN augments HGF-mediated recovery in protamine sulfate (PS)-induced injury in MET knockout podocytes.A and B, using CRIPR-Cas9 MET knockout podocytes were generated. Red fluorescent protein (RFP) is a marker for transfection and confirms the stable knock out of MET post puromycin selection. Western blot for the MET knockout and control podocyte cell lysates. C and D, MET knockout podocytes were transfected with NEPHRIN- and NEPH1-expressing plasmids and then treated with PS and the actin cytoskeletal (green) disorganization was visualized by phalloidin staining. To induce recovery, HGF (50 ng/ml) was added to the PS-treated podocytes. Podocytes transfected with NEPHRIN and NEPH1 showed significantly improved recovery with HGF. Ten cells per experimental condition were evaluated from three experimental trials. The scale bar represents 25 μm. Data are presented as mean ± SEM, and p-values were calculated using the Kruskal–Wallis test one-way analysis of variance. ns, nonsignificant, ∗p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗p ≤ 0.001.