Skip to main content
. 1999 Jul;19(7):4927–4934. doi: 10.1128/mcb.19.7.4927

FIG. 3.

FIG. 3

SV40 large T antigen can counteract the repressive effect of RB on Pol III transcription. Template pVAI (250 ng of DNA) was transcribed by using 2 μl of Mono Q-purified TFIIIB and 2 μl of CHep-1.0 fraction in the presence of 100 ng of either GST (lanes 1 to 3) or GST-RB (lanes 4 to 6) and 20 ng (lanes 2 and 5) or 40 ng (lanes 3 and 6) of immunoaffinity-purified recombinant large T antigen (TAg).