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. 1999 Jul;19(7):4927–4934. doi: 10.1128/mcb.19.7.4927

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Copyright © 1999, American Society for Microbiology

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FIG. 4 — Cl38 and Cl49 cell extracts contain higher TFIIIB activity than A31 cell extracts. (A) Template pVAI (250 ng of DNA) was transcribed by using 2 μl of HeLa-derived PC-C fraction and buffer alone (lane 2) or PC-B fraction (6.4 μg of protein) derived from HeLa (lane 1), Cl38 (lane 3), or A31 (lane 4) cell extracts. (B) Template pVA1 (500 ng DNA) was transcribed by using 4 μl of HeLa-derived PC-C fraction supplemented with 10 ng of recombinant TBP and 6 μl of heat-treated Mono Q-purified TFIIIB (lane 1), buffer alone (lanes 2 and 6), or 7.5 μg of heat-treated extract from Cl38 (lane 3), A31 (lane 4), or Cl49 (lane 5) cells.