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. 1999 Jul;19(7):4927–4934. doi: 10.1128/mcb.19.7.4927

FIG. 5.

FIG. 5

The BRF subunit of TFIIIB coimmunoprecipitates with endogenous RB present in A31 extracts, but this interaction is compromised in extracts of Cl38 and Cl49 cells. (A) Reticulocyte lysate (15 μl) containing in vitro-translated BRF was immunoprecipitated (IP) with anti-RB antibody G3-245 in the presence of buffer alone (lane 2) or 150 μg of whole-cell extract prepared from SAOS2 (lane 3), Cl38 (lane 4), or A31 (lane 5) cells. Proteins retained after extensive washing were resolved on an SDS–7.8% polyacrylamide gel and then visualized by autoradiography. Lane 1 shows 10% of the input reticulocyte lysate containing in vitro-translated BRF. (B) Reticulocyte lysate (15 μl) containing in vitro-translated BRF was immunoprecipitated with anti-RB antibody C-15 in the presence of buffer alone (lane 1) or 150 μg of whole-cell extract prepared from A31 (lane 2) or Cl49 (lane 3) cells. Proteins retained after extensive washing were resolved on an SDS–7.8% polyacrylamide gel and then visualized by autoradiography.