FIG. 6.
The association between endogenous RB and endogenous TFIIIB is greater in A31 cells than in Cl38 and Cl49 cells. (A) Cl38 (lanes 1 and 2) and A31 (lanes 3 and 4) cell extracts (150 μg of protein) were immunoprecipitated (IP) with anti-TAFI48 antibody M-19 (lanes 1 and 3) or anti-RB antibody C-15 (lanes 2 and 4). Precipitated material was resolved on an SDS–7.8% polyacrylamide gel and then analyzed by Western blotting with anti-BRF antiserum 128. (B) Cl49 (lanes 1 and 2) and A31 (lanes 3 and 4) cell extracts (150 μg of protein) were immunoprecipitated with anti-TAFI48 antibody M-19 (lanes 1 and 3) or anti-RB antibody C-15 (lanes 2 and 4). Precipitated material was resolved on an SDS–7.8% polyacrylamide gel and then analyzed by Western blotting with anti-BRF antiserum 128.