FIG. 7.
Transfected HPV E7 activates Pol III transcription in vivo in a manner that is dependent on its RB-binding domain. NIH 3T3 cells were transfected with pVAI (2 μg), pCAT (2 μg), and 6 μg of empty vector (lane 1) or vector encoding wild-type E7 (E7 WT; lane 2), PRO2 mutant E7 (lane 3), Δ21-35 mutant E7 (lane 4), or GLY24 mutant E7 (lane 5). (A) VAI primer extension products. (B) VAI transcription levels that have been normalized to the levels of CAT expression to correct for transfection efficiency and are expressed relative to the value obtained for the empty vector control (designated 1.0).