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. Author manuscript; available in PMC: 2021 Sep 10.
Published in final edited form as: Cell Rep. 2021 Aug 24;36(8):109598. doi: 10.1016/j.celrep.2021.109598

Figure 7. ATAC is required for the expression of translation-related genes.

Figure 7.

(A) Volcano plots representation of differential expression between Supt7l−/−, Yeats2AID/AID, Zzz3AID/AID, Tada3AID/AID, and WT cells. Numbers of significantly misregulated genes are indicated. Ribosome protein genes (RPGs) are highlighted as blue dots.

(B) Heatmap showing log2 FCs observed for all RPGs in Supt7l−/−, Yeats2AID/AID, Zzz3AID/AID, and Tada3AID/AID cell lines.

(C) Intron-containing mRNA levels of three RPGs (Rps24, Rpl29, and Rps5) in Yeats2AID/AID, Zzz3AID/AID, and WT cells upon 2–24 h of IAA treatment were normalized as in Figure 1G. Error bars show SD of 4 biological replicates, using two independent clones. Statistical test performed is Wilcoxon rank-sum test with Benjamini-Hochberg correction for multiple testing.

(D) Heatmap showing log2 FCs observed for four transcription factors involved in the regulation of RPG expression in the indicated cell lines.

(E and F) Western blot analysis of Rpl29 protein levels in two independent Yeats2AID/AID and Zzz3AID/AID cell lines (E) or Supt7l−/− cell lines (F) compared with WT cells. γTubulin serves as loading control.