Figure 6.

Flow cytometric analysis of the cervical lymph nodes

KLN205-MUC1 cells (2 × 10⁵) were implanted in the tongues of DBA/2 mice. After 7 days, G47Δ was intratumorally inoculated into the tongue tumors at a dose of 1 × 10⁶ pfu. After 18 h, the cervical lymph nodes were removed and lymphocytes were analyzed by a flow cytometer. (A) Representative plots of CD69-positive cells among CD8- or CD4-positive T cells. (B) The proportions of CD69-positive lymphocytes among CD8-positive cells (left) and CD4-positive cells (right). A significantly higher proportion of CD69-positive cells among both CD8- and CD4-positive cells was detected in the G47Δ-treated animals compared with mock-treated ones. n = 4 per group. Error bars, ± SEM. ∗p < 0.05 (Student’s t test).