Figure 4.

The positive-feedback loop between Bcl-w and HIF-1α leads to lung cancer malignancy

(A–G) After A549 and H460 cells were transfected with Bcl-w-overexpressing vector or siRNA against HIF-1α (siHIF-1α), indicated cells were analyzed by wound healing assay (A and B) (scale bars, 200 μm), invasion assay using Matrigel-coated Transwells (C and D) (scale bars, 100 μm), sphere forming assay (E and F) (scale bars, 100 μm), and western blot analysis (G). (H–N) After A549 and H460 cells were transfected with HIF-1α-overexpressing vector or shRNA against Bcl-w (shBcl-w), indicated cells were analyzed by wound healing assay (H and I) (scale bars, 200 μm), invasion assay using Matrigel-coated Transwells (J and K) (scale bars, 100 μm), sphere forming assay (L and M) (scale bars, 100 μm), and western blot analysis (N). β-actin was used for normalization in western blot analysis. The data are presented as mean ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Student`s t test.