Figure 2.

Down-regulation of circNFIX repressed HCC cell proliferation and motility. A: qRT-PCR for assessing circNFIX level in HCC cells transfected with si-circNFIX; B: HCC cell proliferation after circNFIX silencing was determined by CCK-8; C, D: The migration and invasion of HCC cells were evaluated by transwell assay (200×); E: The volume of xenograft tumors was measured once a week; F: Tumor weight was detected after the experiment; G: Protein levels of Ki-67 and PCNA in tumor tissues were assessed by Western blotting. **P<0.01; ***P<0.001.