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. 2021 Aug 15;13(8):8697–8710.

Figure 4.

Figure 4

circNFIX acted as a sponge for miR-3064-5p. A: Overlap of the predicted target miRNAs of circNFIX by circBank, starBase and circAtlas; B: qRT-PCR for detecting circNFIX level in HCC and normal tissues; C: Correlation analysis between circNFIX and miR-3064-5p expression in HCC tissues; D: Schematic illustration of the predicted complementary sequences for miR-3064-5p in circNFIX; E: Overexpression of miR-3064-5p in HCC cells was validated by qRT-PCR; F: Relative luciferase activity of HCC cells co-transfected with circNFIX-WT or MUT and miR-3064-5p mimics or mimics NC; G: RNA pull-down assay for evaluating the effect of miR-3064-5p enrichment on circNFIX; H: The recruitment of miR-3064-5p by circNFIX was confirmed by anti-Ago2 RIP assay; I: qRT-PCR for detecting miR-3064-5p expression in circNFIX-silence HCC cells. ***P<0.001.