Figure 7.

circNFIX knockdown restrained HCC cell progression through the miR-3064-5p/HMGA2 axis. A: Overexpression of HMGA2 in HCC cells was verified by Western blotting assay; B: The proliferation was determined by CCK-8 assay; C-E: Cell migratory and invasive capacities were assessed by transwell assay (200×); F-H: The levels of glutamine, glutamate, and α-KGin in HCC cells were measured by commercial kits; I: Western blotting for assessing ASCT2 and GLS1 levels in HCC cells. *P<0.05; **P<0.01; ***P<0.001.