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HG exposure increased circLRP6 expression while decreased HMGA1 expression in VSMCs. (A) CCK-8 assay and (B) EdU staining were employed to estimate proliferation of VSMCs under NG and HG condition. (C and D) Effects of HG exposure on the migratory and invasive abilities of VSMCs were assessed by wound-healing and transwell assays. (E) CircLRP6 level in NG- and HG-treated VSMCs was measured via qRT-PCR. (F) The subcellular localization of circLRP6 in VSMCs was determined by FISH assay.
