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. 2021 Aug 15;13(8):8728–8741.

Figure 7.

Figure 7

miR590-5p targeted FGF-18 in chondrocytes. A: Sequence alignment between miR-590-5p, WT and MUT of 3’-UTR of FGF18. B, C: Chondrocytes were transiently co-transfected with either a WT or mutant FGF18 3’UTR reporter plasmid and either a miR-590-5p mimics or inhibitors. The cells were harvested at 48 h after transfection, and then luciferase activity was measured by dual-luciferase reporter assay. D-F: Relative mRNA and protein expressions of FGF18 after transfection of miR-590-5p mimics or inhibitors were examined by qPCR or WB. *P < 0.05, **P < 0.01 and ***P < 0.001; #P < 0.05, ##P < 0.01 and ###P < 0.001, IL-1β+ mimics or inhibitors versus IL-1β+ NC.