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. 2021 Aug 27;12:701513. doi: 10.3389/fmicb.2021.701513

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Copyright © 2021 Liu, Feng, Wei, Xiao and Zong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alignment of pKPC2_130002 with pKPC2_020019. pKPC2_130002 is a 94.9-kb IncFII plasmid of the Y6:A-:B- type and has bla_KPC–2. pKPC2_130002 is the closest to pKPC2_020019 (accession no. CP028554), an 89.7-kb Y6:A-:B-type bla_KPC–2-carrying plasmid from a K. variicola strain recovered in a hospital of a neighbor city (Meishan, 80 km away from Chengdu) in 2017, with a 94% coverage and 99.93% identity. Comparing with pKPC2_130002, pKPC2_020019 lacks several protein-encoding genes with the products being indicated. On pKPC2_130002 and pKPC2_020019, bla_KPC–2 is located in a non-Tn4401 element containing a transposase-encoding tnpA gene of an unnamed transposon of the Tn3 family at upstream and another tnpA of an unnamed transposon of the TnAs1 family at downstream. This comparison was performed using BRIG (Alikhan et al., 2011) in the default settings.