Skip to main content
. 2021 Aug 27;12:701513. doi: 10.3389/fmicb.2021.701513

FIGURE 4.

FIGURE 4

Alignment of pOXA926_130002 with RHBSTW-00167_2 and p15WZ-82_res. pOXA926_130002 is a 117.8-kb IncF plasmid and has blaOXA–926. Plasmid replication initiation-encoding genes repA of the IncFII replicon and repE of the IncFIA replicon and the conjugation module containing multiple genes (traA to traX and trbB to trbI) are indicated. A gene encoding the transpose of an IS30 family insertion sequence at upstream of blaOXA–926 and a gene encoding a tyrosine recombinase-like integrase at downstream are also shown. pOXA926_130002 is the closest (56% coverage and 97.1% identity) to pRHBSTW-00167_2 (accession no. CP058119), a 223.8-kb K4:A10:B- type IncF plasmid with an additional IncR replicon of an K. michiganensis strain recovered in United Kingdom in 2017. pRHBSTW-00167_2 has no blaOXA–926, while p15WZ-82_res (accession no. CP032357) is another blaOXA–926-carrying plasmid of a K. variicola clinical strain recovered from an unspecified place in China in 2015. p15WZ-82_res belongs to the K4:A-:B- IncF type and also contains a K (pCAV1099-114 type) IncFIB allele. pOXA926_130002 had a 53% coverage and 99.0% identity with p15WZ-82_res.