Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 2;10(9):bio058513. doi: 10.1242/bio.058513

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 2. — Validation of HSPC counts using the Athena Zebrafish Application to detect differences in stem cell populations due to age or genetics of zebrafish embryos. (A) Brightfield and fluorescent images of Tg(itga2b:GFP) embryos at 2-4 dpf and analysed for HSPC count (green) in the tail region (red). (B) HSPC counts at 2 dpf (n=48), 3 dpf (n=54) and 4 dpf (n=47), showing the increase in HSPC number over time. (C) Fluorescent images of the tail of rps14^+/+ and rps14^+/− Tg(itga2b:GFP) at 3 dpf following phz haemolytic stress for 24 h at 24 hpf alongside unstressed controls analysed for HSPC count (green) in the tail region (red). (D) HSPC count of the different conditions, showing no difference in HSPC count between unstressed rps14^+/− (n=27) and unstressed rps14^+/+ (n=22) embryos, but an increase in HSPC count in the phz-stressed rps14^+/+ embryos (n=29) compared with wild type, which does not occur in the phz-stressed rps14^+/− embryos (n=29), from triplicate experiments (Peña et al., 2020 preprint). Statistical analysis using unpaired t-tests. Error bars show mean±s.d. ns, P>0.05; **P<0.01. Scale bars: 500 µm. n refers to the number of embryos analysed for each condition. Cell counts give the Athena cell count for the example image shown.