Figure 3.

Double dose of rF1V enhances protection against Y. pestis challenge. BALB/c mice were vaccinated with two subcutaneous doses, 3 weeks apart with mock/PBS, 0.5 µg, 1.0 µg or 3.0 µg of rF1V (n=10). At 29 days post vaccination, BALB/c mice were exposed to aerosolized bacteria with (A) 4.53x10⁵ CFU (approximately 7 LD₅₀ equivalents) of Y. pestis CO92 or (B) 6.09x10⁵ CFU (approximately 8 LD₅₀ equivalents) of Y. pestis C12, respectively. Survival was monitored for 21 days post challenge.