Figure 4.

Production of IFN-γ by whole splenic lymphocytes from mice vaccinated with rF1V and TLR agonists. BALB/c mice were vaccinated twice, subcutaneously, 3 weeks apart with mock/PBS (n=15), rF1V (n=14), with and without 5 or 25 µg of TLR agonists (n=5). At 28 days post vaccination, mice were euthanized and spleens were harvested. Splenocytes (10⁶ cells) were re-stimulated in the presence of rF1V (25 µg/ml) for 48 hours. The levels of IFN-γ were measured by the Luminex bead-based suspension assay. Log transformed values were analyzed by two-way ANOVA (treatment x assay date) and results are reported as the geometric mean and geometric standard error. p-values are obtained from the main effect contrasts of the ANOVA model. No adjustment has been applied for multiple comparisons. ^†p<0.05, *p<0.01, **p<0.001, ***p<0.0001 (Comparisons show significant differences versus the rF1V (0.5 µg) group).