Table 3.
Effect of Pam3CSK4 (TLR2), Poly(IC)LC (TLR3), MPLA (TLR4), Imiquimod (TLR7), R848 (TLR7/8) and CpG (TLR9) with rF1V vaccine on the F1V antibody response in BALB/c mice.
| Vaccine Treatmentc | Antibody Titera b | Ratio IgG2a/IgG1 | ||
|---|---|---|---|---|
| Class | Isotype | |||
| IgG | IgG1 | IgG2a | ||
| PBS | 50 (1) | 50 (1) | 50 (1) | 1.000 |
| CpG 2006 (25 pg) | 61 (1) | 50 (1) | 63 (1) | 1.260 |
| MPLA (25 μg) | 50 (1) | 50 (1) | 50 (1) | 1.000 |
| Imiquimod (25 μg) | 50 (1) | 50 (1) | 50 (1) | 1.000 |
| Pam3CSK4 (25 μg) | 50 (1) | 50 (1) | 52 (1.08) | 1.040 |
| Poly (IC)LC (25 μg) | 50 (1) | 50 (1) | 50 (1) | 1.000 |
| R848 (25 μg) | 50 (1) | 50 (1) | 50 (1) | 1.000 |
| rF1V (0.5 μg) | 1,326,807 (1.33) | 4,561,855 (1.34) | 19,346 (1.35) | 0.004 |
| rF1V (0.5 μg) + CpG2006 (5 μg) | 2,281,929 (1.12) | 8,865,169 (1.22)* | 479,587 (1.4)*** | 0.054 |
| rF1V (0.5 μg) + CpG2006 (25 μg) | 4,335,445 (1.12)** | 9,332,232 (1.24)* | 1,116,941 (1.24)*** | 0.120 |
| rF1V (0.5 μg) + MPLA (5 μg) | 2,224,093 (1.22) | 6,047,733 (1.21) | 82,080 (1.37)* | 0.014 |
| rF1V (0.5 μg) + MPLA (25 μg) | 1,635,849 (1.21) | 3,622,337 (1.33) | 111,694 (1.39)** | 0.031 |
| rF1V (0.5 μg) + Imiquimod (5 μg) | 2,425,147 (1.17) | 4,215,755 (1.72) | 116,148 (1.72)* | 0.028 |
| rF1V (0.5 μg) + Imiquimod (25 μg) | 3,482,202 (1.27)† | 16,025,318 (1.29)* | 101,113 (1.26)*** | 0.006 |
| rF1V (0.5 μg) + Pam3CSK4 (5 μg) | 4,222,425 (1.17)** | 7,650,820 (1.3) | 45,948 (1.45) | 0.006 |
| rF1V (0.5 μg) + Pam3CSK4 (25 μg) | 2,301,032 (1.16) | 5,047,659 (1.19) | 50,477 (1.31)* | 0.010 |
| rF1V (0.5 μg) + Poly (IC)LC (5 μg) | 1,458,756 (1.5) | 1,527,747 (1.28)* | 105,728 (1.46)* | 0.069 |
| rF1V (0.5 μg) + Poly (IC)LC (25 μg) | 1,918,763 (1.29) | 3,045,846 (1.66) | 132,578 (1.44)** | 0.044 |
| rF1V (0.5 μg) + R848 (5 μg) | 1,098,561 (1.29) | 1,921,799 (1.8) | 87,746 (1.47) | 0.046 |
| rF1V (0.5 μg) + R848 (25 μg) | 3,179,826 (1.14)* | 11,596,475 (1.2) | 139,288 (1.45) | 0.012 |
a
Values represent geometric mean (Geo Mean) with geometric standard error (GSE).
b
All p-values are relative to the rF1V (0.5 μg) control group. †p<0.05, *p<0.01, **p<0.001, ***p<0.0001.
c
Values in parenthesis represent low (5 μg) and high (25 pg) toll-like receptor agonist amount per vaccine dose.