Figure 1.

ADSC-CM inhibited KFB growth and induced KFB apoptosis. (a) Cell growth curve of KFBs in ADSC-CM and control groups (DMEM/F12), n = 6. (b, c, d) Apoptosis was assessed by Annexin V FITC and phycoerythrin staining in mesangial cells, n = 4. (e, f) The mRNA and protein levels of caspase 3 were assessed by RT-PCR and western blotting, respectively, n = 3. (g, h) The mRNA and protein levels of Bcl-2 were assessed by RT-PCR and western blotting, respectively, n = 3. ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 (ADSC-CM vs DMEM/F12). ADSC-CM conditioned medium of adipose-derived stem cells, DMEM Dulbecco’s modified Eagle’s medium, KFBs keloid dermis-derived fibroblasts, OD optical density, F12 Ham’s F-12, Bcl-2 B-cell lymphoma-2, LR viable apoptotic cells, UR non-viable apoptotic cells, UL debris and damaged cells, LL normal cells, FITC Fluorescein isothiocyanate