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. 2021 Sep 3;24(5):764. doi: 10.3892/mmr.2021.12404

Figure 3.

Figure 3.

Knockout of CD36 prevents NF-κB by inhibiting ROS in H9c2 cells. H9c2 cells were incubated with NG (5.6 mM, NG group), HG (30 mM, HG group), HG plus LV3 empty vector (HG + C group), HG plus LV3 containing CD36 (HG + HC group), HG + HC plus MitoTEMPO (100 nM, HG + HC + T group), HG plus LV3 containing the CD36 short hairpin RNA (HG + KC group), and HG plus MitoTEMPO (10 µM, HG + T group) for 72 h. (A and B) The nuclear translocation of NF-κB p65 protein in the nucleus and cytosol was detected by western blotting. (A and C) The IκBα protein expression in cytosolic extracts was measured by western blotting. (D) Immunofluorescence labeling with anti-NF-κB p65 (magnification, ×400). (E) Expression levels of mitochondrial ROS were detected by FACS using MitoSOX reagent. Data are expressed as the mean ± SD. n=4. **P<0.01 vs. NG; #P<0.05 vs. HG. NF-κB, nuclear factor-κB; ROS, reactive oxygen species; HG, high glucose; NG, normal glucose.