Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 3;24(5):764. doi: 10.3892/mmr.2021.12404

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Han et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 4. — Knockout of CD36 ameliorates metabolism reprogramming and lipid accumulation caused by HG in H9c2 cells. H9c2 cells were incubated with NG (5.6 mM, NG group), HG (30 mM, HG group), HG plus LV3 containing CD36 mutant (HG + KC group), HG plus PA (100 µM HG + PA group), or HG + PA plus LV3 containing CD36 mutant (HG + PA + KC group) for 72 h. (A) Protocol used in data collection and calculations for evaluating mitochondrial respiration. (B) Representative OCR curve. (C) Quantitative analysis of non-mitochondrial respiration, basal respiration, ATP production, proton leak, maximal respiration and spare respiratory capacity. (D) Quantitative analysis of glycolysis, glycolytic capacity and glycolytic reserve. All measured OCR and ECAR were normalized by total cellular protein. (E) The triglyceride content in the cells. (F) The p-AMPK and AMPK expression levels were analyzed by western blotting. Data are expressed as the mean ± SD. n=4. **P<0.01 vs. NG, ^#P<0.05 vs. HG. HG, high glucose; OCR, oxygen consumption rate; ECAR, extracellular acidification rate; p-, phosphorylated-.