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. 2021 Sep 3;24(5):763. doi: 10.3892/mmr.2021.12403

Figure 4.

Figure 4.

Sal B improves CHC-induced autophagic dysfunction in RAW264.7 macrophages. The following groups are presented: CONT, untreated control; CHC, 800 µg/ml CHC; CHC + Sal B, 800 µg/ml CHC + 200 µM Sal B; BafA1 + CHC + Sal B, 20 nM BafA1 + 800 µg/ml CHC + 200 µM Sal B; and BafA1, 20 nM BafA1. (A) Western blotting was performed to detect the expression of autophagy-related proteins in RAW264.7 macrophages treated with Sal B and CHCs for 24 h (n=3). (B) Fluorescence microscopy was performed to assess GFP-LC3 autophagic spots in RAW264.7 macrophages treated with Sal B and CHCs for 24 h (scale bar, 200 µm). #P<0.05 vs. untreated control; *P<0.05 vs. CHC. Sal B, salvianolic acid B; CHC, cholesterol crystals; BafA1, bafilomycin A1 inhibitor.