Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 27;11:682596. doi: 10.3389/fonc.2021.682596

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 El-Fadl, Hagag, El-Shafei, Khayri, El-Gedawy, Maksoud, Mohamed, Mohamed, El Halfawy, Khoder, Elawdan, Elshal, Salah and Khalil

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Knockdown efficiency and expression profile of Raf-1, MEK1, LC3B, and ATG12 in siRNA-transfected cells. (A) Relative LDH production from transfected cells upon 2 days posttransfection compared with the control-transfected cells, n = 4. Error bars indicate the SD of four biological replicates (B, C) Relative gene expression of Raf-1 and MEK1, respectively in siRNA-transfected MCF-7 cells upon 48 h posttransfection compared with the control-transfected cells (siRNA anti-Luciferase). (D, E) Relative gene expression of autophagy-related LC3B and Atg12, respectively, indicated by the fold change in siRNA-transfected cells compared with the control-transfected cells. Levels of GAPDH–mRNA were used as an internal control. Error bars indicate the SD of three independent experiments. (F) Quantification analysis of total Raf-1 and lipidated LC3B in the transfected MCF-7 cells indicated by flow cytometry and compared with the control-transfected cells. (G) Quantification analysis of kinetic protein profile of pRaf-1 and pMEK in transfected MCF-7 cells indicated by flow cytometry and compared to control-transfected cells. Data are representative of three independent experiments. A Student’s t-test analysis was performed. **p < 0.01.