FIGURE 2.

A. alternata melanin synthesis during mycelial growth. (A) Pigment formation of A. alternata grown in MM, in MM supplemented with 5.5 mM of L-tyrosine (MMT) and in MM supplemented with 5.5 mM of L-phenylalanine (MMP) after 3 days of growth. (B) Mycelial growth and quantification of the melanin content in A. alternata cells and released in liquid cultures over 10 days. Bars, mycelium dry weight; squares, pigment released into the medium, given as the absorbance at 405 nm (Abs); triangles, cell wall pigment content, measured by determination of the absorbance at 405 nm, as described in section “Materials and Methods”, and given as the absorbance (Abs) per milligram (dry weight) of mycelia (×1,000 for the scale of MM and MMT growth and × 100 for the MMP growth scale).