Figure 6.

Neutral sphingomyelinase in cells and exosomes. (a) Immunoblotting analysis. The position of the 60 kDa was evaluated in relation to the molecular-weight size markers. HaCaT cells were used as positive control [19]; (b) The area density was quantified by Chemidoc Imagequant LAS500 by specific IQ programm. (c) Enzymatic activity of neutral sphingomyelinase. Data are expressed as nU/mg protein/min; (d) Neutral sphingomyelinase activity in relation to enzyme content. Data represent the mean ± SD of three independent experiments performed in duplicate (case number: 6). * p < 0.05 versus the control sample, ^§ p < 0.05 versus cells, CTR, control sample; VD3, vitamin D3 sample.