Figure 2.

Subcellular localization of Orf9b_WT and Orf9b_S53E. Representative immunofluorescence images of Vero E6 cells expressing Orf9b_WT-EGFP or Orf9b_S53E-EGFP 24 h post-transfection (left panels). Gray value profiles of EGFP (green curves) and COXIV (red curves) (right) were measured with Image J (Fiji) software and normalized to the smallest and largest values in each data set. Gray value profiles were plotted along the white dotted lines. Cell nuclei are shown in blue (DAPI) and mitochondria in red. Scale bars, 10 µm.