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. 2021 Sep 1;22(17):9516. doi: 10.3390/ijms22179516

Figure 1.

Figure 1

Generation of primary CD11b+Gr1+CCR9+ macrophages (MAC cells) engineered to overexpress CYB27B1 (MAC-CYP cells) in vitro. The MAC cells were generated as described in Materials and Methods. (A) 4× (left panel) and 40× (right panel) phase bright images of primary MAC cells are shown. (B) A FACS plot shows macrophage markers’ expression, i.e., CD11b and F4/80. (C) A FACS plot shows the expression of CCR9 in the presence or absence of IL-13. Orange line plot: without IL-13, black line plot: with IL-13 (w/IL-13), filled blue plot: isotype control. (D) Schematic diagram of the lentiviral plasmid of lenti-CYP27B1-GFP. SSFV and PGK are promoters. (E) Representative images of MAC (left panel) and MAC-CYP-GFP cells (right panel) under the fluorescence field. (F) A FACS plot shows GFP expression in the MAC and MAC-CYP cells.