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. 2021 Sep 1;22(17):9516. doi: 10.3390/ijms22179516

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Intraperitoneally administered MAC-CYP cells migrated specifically into inflamed colons. (A) Experimental design. Tx: MAC-CYP-GFP cells. (B) Representative 4× images of MAC-CYP-GFP-treated animals show immunostaining of GFP and CD11b of the spleen (a); liver (b); and distal inflamed colons (c). Arrows indicate GFP⁺ cells that have migrated to the proximity of crypts. The boxed area is further enlarged (20×) for viewing the staining of GFP (c1), CD11b (c2), and DAPI (c3). (c4) shows GFP and CD11b merged staining.