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. 2021 Sep 1;22(17):9516. doi: 10.3390/ijms22179516

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Intraperitoneally administered MAC-CYP cells accelerated intestinal stem cell migration in normal healthy animals. (A) Experimental design. (B) Representative images show BrdU staining of non-treated distal colon tissues immediately after BrdU labeling on day 0. Upper panel: 10×; lower panel: 40×. (C) Representative images of distal colon tissues four days after BrdU labeling and three days after the animals were treated with PBS; MAC; or MAC-CYP (day 4). Left panel: 10× images; right panel: 40× images. (D) Average numbers of BrdU⁺ cells of three randomly chosen microscopic fields at the top 1/3 areas of the colon epithelia from PBS-, MAC-, and MAC-CYP-treated animals are shown. * p < 0.05. ANOVA test, n = 5.