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. 2021 Sep 3;22(17):9581. doi: 10.3390/ijms22179581

Figure 1.

Figure 1

Generation of the vascularized bone model. (A) Schematic of the culture conditions and experimental timeline. (B) Viability analyses of the constructs at 7 and 14 days of culture, showing glucose consumption and DNA content. (C) Morphological appearances of constructs after 14 days of culture, showing viable cells positive for green fluorescent protein GFP (MSODs), red fluorescent protein RFP (HUVECs), and Calcein Blue AM (MSOD and HUVEC). Number of experiments = 3, number of replicates/experiment = 4. ** p < 0.005, **** p < 0.00005.