Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 3;22(17):9581. doi: 10.3390/ijms22179581

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effects of the conditioned medium from HPC microcartilage on the vascularized bone model. (A) DNA content analysis to measure cell viability. (B) Biochemical characterizations of the culture supernatant. (C) Quantitative RT-PCR analyses; gene expression is relative to GAPDH levels. ALP = alkaline phosphatase, COL1A1 = collagen type I, OSC = osteocalcin, VEGF = vascular endothelial growth factor, MMP13 = matrix metallopeptidase 13, ADAMTS5 = A disintegrin and metalloproteinase with thrombospondin motifs 5. (D) Characterization of network formation by HUVECs in the MSOD-HUVEC group by imaging and angiogenesis quantification, respectively. Number of experiments = 1, number of replicates/experiment = 4. ns = non-significant, * = p < 0.05, ** = p < 0.005, *** = p < 0.0005, **** p < 0.00005. HPC = HPC microcartilage conditioned medium, Static = conditioned medium from microcartilage not mechanically stimulated.