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. 2021 Aug 25;22(17):9151. doi: 10.3390/ijms22179151

Figure 3.

Figure 3

General growth characteristics of TCC cell lines. (A) Comparison of proliferation ability in cell lines using the MTT assay. All canine TCC cell lines proliferated faster than D17 cells (p = 0.2441, two-way repeated measure ANOVA). KU-CTCC-001 proliferated the fastest. There was no significant difference between KU-CTCC-001 and TCC-001-XDC. Error bars represent SEM. (B) The results of the wound-healing assay. All TCC cell lines filled the wound area within 24 h. Error bars represent SD. Statistical significance was determined by one-way ANOVA; * p < 0.05 (C) Representative images of the colony-forming assay. Error bars represent SEM. Statistical significance was determined by one-way ANOVA; ** p < 0.01. All experiments were performed in triplicate.