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. 2021 Aug 26;22(17):9225. doi: 10.3390/ijms22179225

Figure 5.

Figure 5

Effect of ketamine/melatonin combination after two administrations of melatonin on the expression of Ki67 in the mice hippocampus. Animals (n = 3 per group) were injected at ZT 18 with 4 mg/kg of melatonin (M) or the vehicle (V) as first administration. A second dose of melatonin (M) or the vehicle (V) was administered at ZT 11 in the light cycle. Finally, mice received a third injection of melatonin (4 mg/kg) in combination with a non-effective dose of KET (1.5 mg/kg) at ZT 18 (KM) or solely melatonin (M). Animals were anesthetized and perfused. The brains were sectioned in 30 µm slices and immunostained with an anti-Ki67 antibody and a secondary DyLight 488 antibody. Nuclei were stained with DAPI (A–C). The number of Ki67-positive cells were counted in the sub granular zone in the dentate gyrus (D). Each point represents a determination in one brain slice image. A total of 18 slice images (two images from two different hippocampal zones) derived from 9 brain slices of 3 mice per group were assessed. Data were analyzed using a One-Way ANOVA followed by Tukey post-test, (F(2,48) = 8.943; p = 0.0005) * p < 0.05, *** p < 0.001. Scale bar = 100 µm.