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. 2021 Aug 31;22(17):9485. doi: 10.3390/ijms22179485

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Acute β-PEA administration significantly induced 50-kHz ultrasonic vocalizations (USVs) calls in rats. (A) Experimental timeline for USV test. (B) Representative spectrograms of 50-kHz USVs after acute administration of β-PEA. Black arrows indicate exemplary spectrograms of 50-kHz USVs in rats. (C) Total number of calls of 50-kHz USVs for 30 min after acute administration of β-PEA. (D) Temporal changes in calls of 50-kHz USVs in a 5-min time interval after acute β-PEA administration. * p < 0.05 vs. saline control group. SAL, saline; β-PEA, β-phenylethylamine. n = 8 per group.