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. 2021 Aug 28;22(17):9341. doi: 10.3390/ijms22179341

Figure 7.

Figure 7

RT-qPCR analyses of mRNA of the chondrogenic genes Collagen II (COL II), Aggrecan (ACAN), and cartilage oligomeric matrix protein (COMP) and the dedifferentiation marker gene collagen I (COL I) after differentiation in chitosan flock scaffolds (CFS), alginate hydrogels (ALG), and chitosan/alginate flock scaffolds (CFS+ALG). Relative induction of gene expression was analyzed after 21 days of differentiation relative to GAPDH and human chondrocytes in monolayer culture at the end of expansion in passage 5. Ratios on mRNA levels were calculated from the relative quantification of collagen type II to I (COL II/I). Relative induction of gene expression was analyzed after 21 days of differentiation relative to GAPDH and human chondrocytes in monolayer culture at the end of expansion in passage 5. The results are shown as mean ± SD, n = 3. p-value *** 0.0001–0.001.