Figure 2.

Metformin attenuates cardiac fibrosis in postinfarcted myocardium. Metformin treatment prevents fibrotic remodeling in postinfarcted myocardium. (A) Representative images of Sirius Red staining of frozen heart tissue sections from vehicle- or metformin-treated mice subjected to 30 min of cardiac ischemia and 14 days of reperfusion. Metformin treatment (5 mg/kg, i.p.) started 15 min after the onset of reperfusion and continued for 14 days. (B) Quantification of fibrotic area from (A), n = 4–5. Data are presented as mean ± SEM. One-way ANOVA followed by Bonferroni’s post hoc test: ** p < 0.01 vs. control (C), § p < 0.001 vs. I/R.