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. 2021 Aug 29;22(17):9368. doi: 10.3390/ijms22179368

Figure 1.

Figure 1

Dose dependent effects of grapefruit IntegroPectin on neuronal cell viability. (a) Cell viability of Integropectin treatment (24 h) in dose dependent experiment (n = 36); (b) Representative morphological images of untreated cells (Ctrl) or cells treated with different doses (1, 10 mg/mL) of IntegroPectin (24 h); (c) Cytotoxicity associated to IntegroPectin treatment (1 mg/mL, 24 h), evaluated by fluorescence developed using CellTox Green Cytotoxicity Assay (n = 13). Positive control Ctrl (+) is represented by cell exposure to lysis buffer; (d) Microscopy inspection of DAPI-labeled nuclei in Ctrl cells or treated with IntegroPectin (1 mg/mL, 24 h). Related bright field and merged pictures are also shown. Tukey test: ### p < 0.001 as compared to control (Ctrl) group; *** p < 0.001. Scale bar 50 µm.