Figure 4.

Effects of IntegroPectin on ROS production driven by exposure to aqueous H₂O₂. (a) DCFH-DA, DAPI and merged fluorescence microscopy images of untreated cells (Ctrl) or treated with In-tegroPectin or with H₂O₂ alone or in combination with IntegroPectin; (b) Histogram of fluores-cence intensity of untreated cells (Ctrl) or treated with IntegroPectin or with H₂O₂ alone or in combination with IntegroPectin measured using DCFH-DA fluorescence assay (n = 26); (c) Oxida-tion kinetics of untreated cells (Ctrl) or treated with IntegroPectin or with H₂O₂ alone or in com-bination with IntegroPectin, monitored using DCFH-DA fluorescence assay. Scale bar: 50 μm. Tukey test: ### p < 0.001 as compared to control (Ctrl) group, *** p < 0.001.