Table 1.
List of antisera used for immunostaining. 5-HT = serotonin (5-hydroxy-tryptamin), AR = androgen receptor, AVP = arginine-vasopressin, Cry = cryptochrome, DBH = dopamine beta-hydroxylase (the guiding enzyme of norepinephrine synthesis), GAD-65/67 = glutamic acid decarboxylase (the guiding enzyme of GABA synthesis), GRP = gastrin-releasing peptide, ME = met-enkephalin, NPY = neuropeptide Y, OR = estrogen receptor, OXY = oxytocin, PER = Period, SOM = somatostatin, SP = substance P, TH = tyrosine hydroxylase (the guiding enzyme of dopamine synthesis), VIP = vasoactive intestinal polypeptide.
| Antibody against | Working Dilution | Producer |
|---|---|---|
| AVP | 1:1000 | Own production [29] |
| VIP | 1:1000 | Sigma product, product number: V3508 |
| GRP | 1:500 | Affinity Res. Prod., Exeter, UK |
| GAD-65/67 | 1:1000 | Biotrend Chemikalien GmbH, Köln, Germany |
| 5-HT | 1:1000 | Diasorin, Stillwater, MN, USA |
| SP | 1:1000 | Chemicon International, Temecula, CA, USA |
| NPY | 1:1000 | Affinity Res. Prod., Exeter, UK |
| ME | 1:1000 | Chemicon International, Temecula, CA, USA |
| OXY | 1:1000 | Own production [32] |
| SOM | 1:1000 | Chemicon International, Temecula, CA, USA |
| AR | 1:50 | Novocastra Laboratories, Newcastle, UK |
| OR | 1:50 | Novocastra Laboratories, Newcastle, UK |
| TH | 1:1000 | Chemicon International, Temecula, CA, USA |
| DBH | 1:1000 | Chemicon International, Temecula, CA, USA |
| Clock | 1:60 | Santa Cruz Biotechnology, Inc., Dallas, TX, USA |
| Per 1 | 1:60 | Santa Cruz Biotechnology, Inc., Dallas, TX, USA |
| Per 2 | 1:60 | Alpha Diagnostics Ltd., Reinach, Switzerland |
| Cry 2 | 1:60 | Alpha Diagnostics Ltd., Reinach, Switzerland |