Figure 2.
Population doubling times (PDTs) of MDA-MB-231 and SK-BR-3 cells treated with different concentrations of eugenol. The cells were seeded (2 × 105 cells/well) in an E-16 plate and continuously observed for 72 h, during which time the cell index values were measured. Cell proliferation was observed at 15-min intervals. Each data point signifies an average value ± SD. All cells were analyzed in triplicate. *, #, $, and & indicate significant changes when compared to the control condition when 4 μM, 8 μM, and 5 μM of eugenol were applied, respectively (p < 0.05).