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. 2021 Aug 26;22(17):9243. doi: 10.3390/ijms22179243

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of eugenol treatment on LC3 and p62 mRNA and protein expression levels. MDA-MB231 and SK-BR-3 cells were treated for 24 h (A,B). Thereafter, LC3 and p62 mRNA expression levels were quantified by RT-PCR. The protein expression levels of LC3 and p62 were then assessed in MDA-MB231 and SK-BR3 cells treated for 24 h with 0, 4, and 8 μM and 0, 5, and 10 μM eugenol, respectively (C,D). * indicates a significant change compared to untreated cells (0 μM) at p < 0.05 based on a one-way ANOVA and the Tukey–Kramer post-ANOVA test.