Figure 5.

L-sepiapterin treatment restored endothelial nitric oxide synthase function. 4C11+ metastatic melanoma cells were treated or not (non-treated) with 40 μM L-sepiapterin (L-SEP) for two hours, and superoxide anion levels were analyzed using (A) DHE by HPLC or (B) flow cytometry, and nitric oxide amount were evaluated by (C) NO analyzer or by (D) flow cytometry using DAF. Values are reported in the bar graphs and expressed as the means ± S.D. The experiments were performed in triplicate and p values were based on the one-way ANOVA test followed by Bonferroni post-test; ***, p < 0.001.