Figure 7.

In vivo melanoma growth was impaired by endothelial nitric oxide synthase downregulation. 4C11+ metastatic melanoma cells were subcutaneously inoculated into C57BL/6 mice and (A) tumor weight and (B) volume were determined. WT: wild type 4C11+ metastatic melanoma cell line; TRC1: control non-target shRNA; sheNOS#1 and wheNOS#2: clones silenced for eNOS. Values are reported in the bar graphs and expressed as the means ± S.D. The experiments were performed in triplicate and p values were based on the one-way ANOVA test followed by the Bonferroni post-test or two-way ANOVA test followed by Bonferroni post-test; *, p < 0.05, ***, p < 0.001.