Figure 1.

Experiment fixation scheme: overview over experimental conditions from which samples were acquired. Transcriptomics datasets are indicated in green, and high-throughput chromatin conformation capture (Hi-C) datasets are shown in orange. External datasets are labeled as such. (a) During the 23rd DLR (Deutsches Zentrum für Luft- und Raumfahrt) parabolic flight campaign (PFC) Jurkat cell samples were fixed shortly before initiating the first parabola (1gIF, compare Figure 1), at the end of the hypergravity phase (BL PFC hyp-g), and after the end of the subsequent first zero gravity phase. A ground sample which was inside the flight hardware but was not exposed to altered gravity was taken as a hardware ground control. (b) During the TEXUS-51 sounding rocket mission, the rocket payload was accelerated by two rocket engine stages for 75 s. At the end of the acceleration phase, the BL-TX hyp-g sample was fixed to monitor the hypergravity and vibrational effects. After another 300 s, the samples exposed to microgravity and samples spinning on an onboard 1× g centrifuge were fixed. A hardware 1× g ground control and a cell culture control were taken 15 min after the rocket launch. (c) During the Jurkat ground-based facilities campaign, the samples were exposed to vector-averaged gravity on a pipette clinostat spinning at 60 rotations per minute as a simulation of microgravity and to a 9× g pipette centrifuge to provide hypergravity. The samples were fixed after 5 min. A static sample as a control was fixed in parallel that was aspirated to pipettes but was only exposed to vibration at the clinostat base plate. (d) Control groups were acquired during the U937 ground-based facilities campaign. The BL sample was fixed immediately after being filled into pipettes, and the 1× g ground-based facility (GBF) samples stayed in pipettes at 1× g for 300 s before fixation. A cell culture control was taken in parallel. (e) External control experiment 2 (GSE98694) based on human immune-derived cells that were analyzed on the same microarray (AffyMetrix HTA2.0) as all other experiments. Three sample groups were included in the datasets, slan+ cells that were analyzed in the original study, CD1c+ reference cells (sample group 2), and CD141+ reference cells (sample group 3). (f) During the 4th Swiss Parabolic Flight Campaign (Swiss PFC), the samples were acquired from the same conditions as from the 23rd DLR PFC campaign. Here, chromosome conformation capture samples (Hi-C) were generated by crosslinking the samples with 3% formaldehyde and subsequently quenching the crosslinking reaction after a 3 min incubation time with glycine.