Table 1.

Datasets used in the study, including the organism’s name, duration of spaceflight or unloading, assay type, sex, strain, tissue, age, and sample size. Refer to GeneLab and GEO databases for further details. GC: ground control, FLT: spaceflight, VC: vivarium control, HU: hindlimb unloading, Gastroc: gastrocnemius, Quad: quadriceps, VL: vastus lateralis, F: female, M: male, Mx: mixed; H: hermaphrodite. Tg: Gal4-UAS transgenic line expressing two copies of eGFP from the hemolectin promoter. N: number of individuals, rep: replicate of pooled samples run for transcriptomic analysis. Refer to [24,25,37,38,39,40,41,42,43] for links to GeneLab processed data and versions used in this study.

Dataset	Organism	Duration	Vehicle	Assay	Sex	Strain	Tissue	Age/Stage	Sample Size
GLDS-104	M. musculus	37 d	ISS	RNAseq	F	C57BL/6J	Soleus	16 wks	N = 6 (GC); N = 6 (FLT)
GLDS-99	M. musculus	37 d	ISS	RNAseq	F	C57BL/6J	EDL	16 wks	N = 6 (GC); N = 6 (FLT)
GLDS-21	M. musculus	11 d, 19 h	STS-108	Microarray	F	C57BL/6J	Gastroc	9 wks	N = 4 (GC); N = 4 (FLT)
GLDS-21	M. musculus	12 d	N/A (HU)	Microarray	F	C57BL/6J	Gastroc	9 wks	N = 5 (VC); N = 5 (HU)
GLDS-103	M. musculus	37 d	ISS	RNAseq	F	C57BL/6J	Quad	16 wks	N = 6 (GC); N = 6 (FLT)
GLDS-370/GEO GSE24215	H. sapiens	10 d	N/A (Bedrest)	Microarray	M	N/A	VL	24-27 yrs	N = 10 (Longitudinal)
GLDS-3	D. melanogaster	12 d, 18.5 h	STS-121	Microarray	Mx	Tg	Whole organism	3rd instar larvae	N = 50/rep × 6 (GC); N = 50/rep × 6 (FLT)
GLDS-3	D. melanogaster	12 d, 18.5 h	STS-121	Microarray	F	Tg	Whole organism	Adults	N = 20/rep × 3 (GC); N = 20/rep × 3 (FLT)
GLDS-113	C. elegans	10 d	ISS	Microarray	H	N2	Whole organism	Mixed stage	N ≈ 10000/rep × 3 (GC); N ≈ 10000/rep × 3 (FLT)