Figure 5.

Apoptotic effect of farrerol (FA) in SKOV3 was mediated by the ERK MAPK pathway. (a) Expression levels of MAPK in FA-treated SKOV3 cells were detected using Western blotting. (b) The viability of SKOV3 cells following treatment with each MAPK inhibitor with or without FA (160 μM) was measured via an MTT assay. (c) Caspase-3 activity of SKOV3 cells following treatment with each MAPK inhibitor with or without FA (160 μM), as measured by a colorimetric Caspase-3 activity assay. (d) Expression levels of PARP, cleaved PARP, ERK, and phospho-ERK in FA-treated SKOV3 cells with or without PD98059, as detected using Western blotting. (e) Expression levels of the cleaved PARP/PARP ratio. (f) Expression levels of the p-ERK/ERK ratio. All data are means ± standard deviation from three independent experiments.