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. 2021 Aug 30;22(17):9400. doi: 10.3390/ijms22179400

Figure 6.

Figure 6

Farrerol (FA) reverses TNF-α induced lipid wasting in differentiated 3T3-L1 cells. (a) Differentiated 3T3-L1 cells were treated with TNF-α (20 ng/mL) with or without FA (15 or 30 µM) for 48 h. The representative images of the Oil Red O (ORO)-stained-cells in each indicated group are shown. (b) The ORO staining in the cells was dissolved in Propan-2-ol and measured. (c) Expression levels of PGC1a, UCP-1, and PPARγ in differentiated 3T3-L1 cells treated with TNF-α with or without FA for 48 h were detected using Western blotting. (d) The expression levels of PGC1a, UCP-1, and PPARγ are shown. Equal loading was confirmed by the quantification of β-actin. Data represent the means ± standard deviation from three independent experiments.