Figure 7.

Effects of farrerol (FA) on lipid accumulation, triglyceride content, and adipogenic differentiation in 3T3-L1 cells. (a) First, 3T3-L1 preadipocytes were treated with differentiation medium in the presence or absence of FA at 15 or 30 μg/mL for 8 days. Lipid accumulation was quantified by staining with Oil Red O (ORO) solution and absorbance was measured at 495 nm. Differentiated 3T3-L1 cells photographed at 100× magnification after ORO staining. (b) Quantification of ORO-stained intracellular lipid content. (c) Intracellular triglyceride content quantified by a triglyceride assay kit and assessed at 570 nm. Preadipocytes were used as negative controls (NC), whereas fully differentiated adipocytes were used as positive controls (PC). (d) Cell viabilities were determined with an MTT assay. (e) The 3T3-L1 preadipocytes were treated with FA at the indicated concentrations for 24 or 48 h. The relative mRNA expression levels of PPARγ, C/EBPα, adiponectin, HSL, and LPL were assessed by real-time PCR. Each experiment was repeated in triplicate. Bars represent means ± standard error (*, p < 0.01 compared to control group).